Droplet Surfactant for PCR applications

PCR - Application Emulseo

Polymerase Chain Reaction (PCR) is a technic that allows to make multiple duplicates of a DNA or RNA sample (up to several tens of thousands).

By artificially increasing the amount of samples, it simplifies the identification of targeted sequences in the samples.

Scientists developed probes to detect these sequences amidst the whole sample but they could not detect sequences present at under 10%.

Droplet-based microfluidic allowed a huge improvement of this technology. With drople-based microfluidics it is now possible to detect sequences present at under 0,01%.

There are numerous applications that can benefit from this improvement going from mutation detection in research to cancer monitoring in hospital.

This major improvement can only be achieved by using droplets that are stable through all the PCR workflow (usually 2 hours run and frequent changes of temperature up to 95°C) and that is what FluoSurf is made for.
Its unique features allow it to keep emulsions stables while preserving the precious samples encapsulated.