Digital Droplet PCR
Digital Polymerase Chain Reaction (PCR) is a highly precise technique used to make multiple copies of small target DNA fragments to a large enough amount to facilitate their detection and quantification.
With digital droplet PCR, samples are partitioned into thousands of individual reactions of defined volume inside water-in-oil microdroplets. PCR amplification of the template nucleic acids occurs in each droplet with high sensitivity. Droplets are individually analyzed and scored as positive (fluorescent droplet containing target sequence) or negative (non-fluorescent). Poisson statistics are then applied to the fraction of positive droplets to calculate the absolute concentration of the template nucleic acids.
Some of the advantages of this technology in comparison with standard quantitative PCR include the possible detection of rare events, copy number variation, and absolute quantification with high precision and sensitivity.
Our surfactants allow to keep droplets stable throughout the whole PCR workflow while preserving the integrity of their content.
Streamlined digital bioassays with a 3D printed sample changer
Bacterial expression systems for enzymatic activity in droplet based microfluidicsScientific paper
Variable inter and intraspecies alkaline posphatase activity within single cells of revivied dinoflagellatesScientific paper
A droplet-based microfluidic flow cytometry enabling absolute quantification of single-cell proteins leveraging constriction channelScientific paper
Confining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivationScientific paper
Active single cell encapsulation using SAW overcoming the limitations of Poisson distribution.